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Daphnia

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renoufk View Drop Down
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    Posted: 19 Jan 2005 at 9:35am
I am using Daphnia to measure heart rate in my Biology coursework, how do you look after them ,what do you feed them? Thanks
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renoufk View Drop Down
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Post Options Post Options   Thanks (0) Thanks(0)   Quote renoufk Quote  Post ReplyReply Direct Link To This Post Posted: 20 Jan 2005 at 1:34pm
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jopedder View Drop Down
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Post Options Post Options   Thanks (0) Thanks(0)   Quote jopedder Quote  Post ReplyReply Direct Link To This Post Posted: 24 Jan 2005 at 12:52pm

I did an experiment measuring daphnia heart rates when I was at school - I was looking at effects of poisons (caffiene, alchohol and nicotine) on heart rates.  If this is what you are doing as well take note that at higher concentrations nicotine appeared to kill the daphnia and they had no heart beat, so on these runs I discarded the daphnia into a seperate container, only to find out that they had just been stunned and there hearts started working agoin after a few minutes! - this meant that I had to re-do some of the runs taking this into account.

Also remember that as you examine the daphnia under a microscope they will heat from the observation light, increasing heart rate.

Good Luck!

Jo

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Post Options Post Options   Thanks (0) Thanks(0)   Quote renoufk Quote  Post ReplyReply Direct Link To This Post Posted: 27 Jan 2005 at 1:48pm

To Jo,

Thank you that is ideal, yes I am measuring the effect of caffeine on the heart rate. Could you possibly tell me the concentration you used of caffeine, as I've seen on a site to use 1% caffeine in water, but am not sure yet how much total volume of water and therefore caffeine should be used- how much did you use per daphnia?!

Thanks

Katie

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jopedder View Drop Down
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Post Options Post Options   Thanks (0) Thanks(0)   Quote jopedder Quote  Post ReplyReply Direct Link To This Post Posted: 28 Jan 2005 at 2:18pm

Sorry Katie,

It was about 8 years ago that I did the experiment, and I no longer have my notes.  However my mum works in a school prep lab, so I am forwarding your posts onto her to see if she can help.

The 1% will be the proportion of caffeine in water, i.e. 99 ml water, 1ml caffeine, therefore the total volume of water is irrelevant.

Jo

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