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adder skin needed for molecular analysis

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magdawu View Drop Down
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    Posted: 02 Jun 2015 at 11:35am
Dear All,

I am writing to you to ask if you maybe have a piece of dead adder skin found on the road or elsewhere. I am doing bioinformatic analysis of potential antimicrobial peptides in newly released Vipera berus DNA sequences - this is my MSc project. However, because I found some potential peptide sequences, I would like to try to extract it from adder's DNA and test it for its antimicrobial properties. I need just a small piece, up to several sq cm.

I would appreciate your help!

Magda
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will View Drop Down
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Post Options Post Options   Thanks (1) Thanks(1)   Quote will Quote  Post ReplyReply Direct Link To This Post Posted: 03 Jun 2015 at 8:57am
just to clarify - you need actual dead adder fragment rather than sloughed skin?
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magdawu View Drop Down
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Post Options Post Options   Thanks (0) Thanks(0)   Quote magdawu Quote  Post ReplyReply Direct Link To This Post Posted: 04 Jun 2015 at 1:50pm
Hi Will! Thank you for your reply! It actually can be both, sloughed skin should have enough DNA for the analysis I need. Do you have one?
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Post Options Post Options   Thanks (0) Thanks(0)   Quote will Quote  Post ReplyReply Direct Link To This Post Posted: 04 Jun 2015 at 4:33pm
I'll have a look - just confirming that sloughs were OK.  I saw lots earlier in the season when the males were shedding, but I may be able to find some at my sites.
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Post Options Post Options   Thanks (0) Thanks(0)   Quote Rags Quote  Post ReplyReply Direct Link To This Post Posted: 04 Jun 2015 at 5:45pm
Hi Magda, I think ARC have a stock pile of shed reptile skins. Drop them an e-mail.
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will View Drop Down
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Post Options Post Options   Thanks (0) Thanks(0)   Quote will Quote  Post ReplyReply Direct Link To This Post Posted: 04 Jun 2015 at 6:57pm
that's a very good point - when we were turning tins for smooth snakes a couple of weeks ago Ben Limburn from ARC was assiduously collecting any fragments of slowworm and smooth snake sloughs, and I guess they would do the same for adder sloughs as well!
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